Transient Expression of Strictosidine Synthase in Tobacco Leaves by Vacuum Infiltration
WANG Miao*, LI Qiu-Rong
(Institute of Applied Ecology, the Chinese Academy of Sciences, Shenyang 110016 , China; 1General Hospital in Shenyang Military District, Shenyang 110016, China )
Abstract
Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves.
Key
words strictosidine synthase; vacuum infiltration; subcellular compartments;
Nicotiana tabacum
Corresponding author: Tel,86-24-23916282; Fax, 86-24-23843313; e-mail,[email protected]